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Viewing cable 06ZAGREB236, CROATIA: AVIAN INFLUENZA UPDATE: FEBRUARY

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Reference ID Created Released Classification Origin
06ZAGREB236 2006-02-22 15:44 2011-08-25 00:00 UNCLASSIFIED Embassy Zagreb
VZCZCXRO5335
RR RUEHAG RUEHDA RUEHDF RUEHFL RUEHIK RUEHKW RUEHLA RUEHLN RUEHLZ
RUEHROV RUEHSR RUEHVK RUEHYG
DE RUEHVB #0236 0531544
ZNR UUUUU ZZH
R 221544Z FEB 06
FM AMEMBASSY ZAGREB
TO RUEHC/SECSTATE WASHDC 5736
INFO RUEHZL/EUROPEAN POLITICAL COLLECTIVE
RUEHPH/CDC ATLANTA GA
RUEAUSA/DEPT OF HHS WASHDC
RUERAFX/DIR AFMIC FORT DETRICK MD
RUEKJCS/SECDEF WASHDC
RUCPDOC/USDOC WASHDC
RUEHRC/USDA WASHDC
UNCLAS ZAGREB 000236 
 
SIPDIS 
 
SIPDIS 
 
DEPT PLEASE PASS TO DS/DSS, DS/IP, M/MED/JCTRIPLETT 
FAS/CMP/DLP WETZEL AND MAGGINNIS 
FAS/ITP/EAMED POMEROY AND FLEMING 
CA/OCS/ACS DAVID SCHENSTED 
USAID FOR E&E/ECA/B/ANNE CONVERY 
 
E.O. 12958: N/A 
TAGS: TBIO SENV ECON EAGR PREL CASC HR
SUBJECT: CROATIA: AVIAN INFLUENZA UPDATE: FEBRUARY 
22 
 
REF: ZAGREB 0222 AND PREVIOUS 
 
1. (U) SUMMARY:  On February 21, the Poultry Center 
lab at the Croatian Veterinary Institute confirmed 
that a dead swan found on the island of Ciovo tested 
positive for H5N1.  The lab continues to operate 
without a real-time polymerase chain reaction (RT- 
PCR) machine but a replacement machine is expected 
in March.  The discovery of the infected swan has 
not activated any of Embassy Zagreb's tripwires. 
END SUMMARY 
 
-------------------------------------------- 
H5N1 NOT UNEXPECTED, BUT LOCATION IS UNUSUAL 
-------------------------------------------- 
2.  (U) Ciovo is located near the Split airport on 
the Dalmatian coast and is not a common area for 
migratory swans.  Ministry of Agriculture officials 
speculated that the bird died in flight, fell into 
the sea and washed up in Ciovo.  The amount of 
poultry in the Ciovo area is negligible and the GOC 
has started implementing biosaftey measures. 
 
----------------------------------- 
LESS THAN OPTIMAL CONDITIONS IN LAB 
----------------------------------- 
3.  (U) Emboffs visited the Poultry Center lab and 
met with the director, Dr. Vladimir Savic, on 
February 22.  Since the removal of the RT-PCR 
machine, Savic said they are isolating the virus in 
chicken embryos, testing for H5 or H7 antisera and 
then using a conventional PCR machine to test for 
the N1 gene.  The final step, DNA sequencing of the 
virus, is done at the Ruder Boskovic Institute. 
Savic said that, other than the initial sample in 
October, samples are not sent to the EC reference 
lab for confirmation. 
 
4.  (U) Savic conceded that although the Poultry 
Center operates a bio-safety level (BSL) 1 
laboratory, the lab employs testing methods (of 
isolating the virus in chicken embryos) that should 
be done in a higher BSL lab.  Using a RT-PCR instead 
of chicken embryos would negate the need for a 
higher BSL lab, however, as a self-funded entity, 
the Poultry Center cannot afford to buy the machine. 
The Center is currently waiting for payment by the 
GOC for the tests it has already completed (over 
4000 since September 2005).  A Ministry of 
Agriculture representative told Savic that the GOC 
would purchase a RT-PCR machine within the next few 
months and that payment for the Center's services 
would be forthcoming.  In the mean time, Savic has 
arranged for a free trial-period lease of a RT-PCR 
machine from a company called Applied Bio Systems. 
The Applied Bio Systems machine should be installed 
sometime in March. 
 
5.  (U) The latest discovery of a swan infected with 
H5N1 has not activated any of Embassy Zagreb's 
tripwires.  The consular section has posted the 
latest Avian Flu Fact Sheet on the announcements 
section of the Embassy's webpage.  Consular staff 
will also provide avian flu informational materials 
and answer questions at four upcoming town hall 
receptions for American citizens, hosted by the 
Ambassador, in major Croatian cities. 
 
FRANK